IRS-1 (phospho Ser616) rabbit pAb

• Catalog Number: orb768802
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IRS-1 (phospho-Ser616)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser612. AA range:578-627
• MW: 170
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-IRS1 antibody, anti-Insulin receptor substrat
• Note: For research use only

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRS-1 (Phospho-Ser612) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IRS-1 (Phospho-Ser612) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HUVEC cells treated with insulin 0.01U/ml 30', using IRS-1 (Phospho-Ser612) Antibody. The lane on the right is blocked with the phospho peptide.