IRF9 Antibody / Interferon regulatory factor 9

• Catalog Number: orb2636557
• Category: Antibodies
• Description: Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-IRF9-2F8
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Recombinant full-length human IRF9 protein was used as the immunogen for the IRF9 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This IRF9 antibody is available for research use only.
• UniProt ID: Q00978
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the IRF9 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells using IRF9 antibody (green, clone PCRP-IRF9-2F8) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with IRF9 antibody (blue, clone PCRP-IRF9-2F8), and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free IRF9 antibody (clone PCRP-IRF9-2F8) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using IRF9 antibody (clone PCRP-IRF9-2F8). These results demonstrate the foremost specificity of the PCRP-IRF9-2F8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.