IRF8 Antibody

• Catalog Number: orb1523734
• Category: Antibodies
• Description: IRF8 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Mouse
• Immunogen: Between 374 and 424
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: IRF8
• UniProt ID: P23611
• NCBI: NP_032346.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: interferon regulatory factor 8; IRF-8; interferon
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse IRF8 by western blot. Samples: Whole cell lysate (50 µg) from L1210cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IRF8 antibody orb1523734 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse IRF8 by western blot. Samples: Whole cell lysate (15 and 50 µg) from RAW 274.7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IRF8 antibody orb1523734 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human IRF8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RAW 274.7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IRF8 antibody orb1523734 used for IP at 3 µg per reaction.