IRF7 (phospho-Ser471/472) antibody

• Catalog Number: orb6233
• Category: Tools
• Description: IRF7 (phospho-Ser471/472) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human IRF7 around the phosphorylation site of Ser471/472 (GV(p-S)(p-S)LD)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, ICC=1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: Phospho-IRF7 (Ser471 + Ser472)
• UniProt ID: Q92985
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Interferon regulatory factor 7; Interferon regulat
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraffin-embedded mouse pancreas tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.).IRF7 (phospho-Ser471/472) antibody at 1:200 dilution with overnight incubation

Paraffin-embedded rat spleen tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.).IRF7 (phospho-Ser471/472) antibody at 1:200 dilution with overnight incubation at

Western blot analysis of rat kidney lysates(Lane 1, 30ug) and rat brain lysates(Lane2, 30ug) using IRF7 (phospho-Ser471/472) antibody