IRF3 Antibody

• Catalog Number: orb1524929
• Category: Antibodies
• Description: IRF3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Mouse
• Immunogen: between 375 and 419
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:2,500; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: IRF3
• UniProt ID: P70671
• NCBI: NP_058545.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: IRF-3; interferon regulatory factor 3; C920001K05R
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse IRF3 by western blot and immunoprecipitation. Samples: Whole cell lysate from mouse bone marrow dendritic (D; 50 and 15 µg for WB; 1 mg for IP, 20% of IP loaded) , and NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-IRF3 antibody orb1524929 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. IRF3 was also immunoprecipitated by rabbit anti-IRF3 antibody, which recognizes a disparate epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).