IRAK4 (phospho-Thr345) antibody

• Catalog Number: orb6226
• Category: Tools
• Description: IRAK4 (phospho-Thr345) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IRAK4 around the phosphorylation site of Thr345 (VM(p-T)SR)
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1ug/Test, WB=1:500-2000, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 51 kDa
• Target: phospho-IRAK4 (Thr345)
• UniProt ID: Q9NWZ3
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: IRAK4 (phospho T345); p-IRAK4 (phospho T345); IL-1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraffin-embedded rat brain tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). IRAK4 (phospho-Thr345) antibody at 1:200 dilution with overnight incubation at 4 d