IRAK3 Antibody

• Catalog Number: orb1239522
• Category: Antibodies
• Description: IRAK3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: IRAK-M antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRAK-M.The immunogen is located within the last 50 amino acids of IRAK-M.
• Concentration: 1 mg/mL
• Dilution range: IRAK-M antibody can be used for detection of IRAK-M by Western blot at 1 μg/mL. IRAK-M antibody can also detect IRAK-M by immunohistochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in human and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 66 kDa Observed: 70 kDa
• Target: IRAK3
• UniProt ID: Q9Y616
• NCBI: NP_009130
• Storage: IRAK-M antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IRAK-M Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IRAK-M Antibody: ASRT5, IRAKM, Interleukin-1 recep
• Note: For research use only
• Application notes: IRAK-M antibody can be used for detection of IRAK-M by Western blot at 1 μg/mL. IRAK-M antibody can also detect IRAK-M by immunohistochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in human and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IRAK-M in (M) mouse spleen and (R) rat liver tissue lysates with IRAK-M antibody at 1 µg/mL.

Immunohistochemistry of IRAK-M in human spleen tissue with IRAK-M antibody at 5 µg/mL.

Immunofluorescence of IRAK-M in Rat Liver tissue with IRAK-M antibody at 10 µg/mL.

Immunohistochemical staining of rat liver tissue using IRAK-M antibody at 2 µg/mL.

Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 20 µg/mL. Red: IRAK-M Antibody (orb1239522) Blue: DAPI staining.