IPO8 Antibody

• Catalog Number: orb1247363
• Category: Antibodies
• Description: IPO8 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SAFNFGTVPSNN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Tonsil shows cytoplam / perinuclear staining in the parenchyma. Recommended concentration, 4-6ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: IPO8
• UniProt ID: O15397
• NCBI: NP_001177924.1, NP_006381.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: IPO8, importin 8, RANBP8, RAN-binding protein 8, F
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Tonsil shows cytoplam / perinuclear staining in the parenchyma. Recommended concentration, 4-6ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247363 (4 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. Similar results were obtained after antigen retrieval at pH9.