IP3R1 Antibody

• Catalog Number: orb1527111
• Category: Antibodies
• Description: IP3R1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 2708 and 2758
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.; IHC-IF - 1:50 - 1:250
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: IP3R1
• UniProt ID: Q14643
• NCBI: Q14643.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: IP3R1; inositol 1,4,5-trisphosphate receptor type
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human IP3R1 by western blot. Samples: Whole cell lysate (5, 15, and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-IP3R1 antibody orb1527111 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human IP3R1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-IP3R1 antibody orb1527111 used for IP at 3 µg/mg lysate.

Detection of mouse IP3R1 by immunohistochemistry.Sample: FFPE section of mouse cerebellum. Antibody: Affinity purified rabbit anti-IP3R1 (Cat. No. orb1527111) used at a dilution of 1: 200 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Detection of mouse IP3R1 by immunohistochemistry.Sample: FFPE section of mouse cerebellum. Antibody: Affinity purified rabbit anti-IP3R1 (Cat. No. orb1527111) used at a dilution of 1: 100 (2µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100. Counterstain: DAPI (blue).