Ionotropic Glutamate receptor 4/GRIA4 Antibody

• Catalog Number: orb215961
• Category: Antibodies
• Description: Ionotropic Glutamate receptor 4/GRIA4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human GRIA4 recombinant protein (Position: Q176-K350). Human GRIA4 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 100871 MW
• UniProt ID: P48058
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Glutamate receptor 4;GluR-4;GluR4;AMPA-selective g
• Note: For research use only
• Application notes: WB: The detection limit for GRIA4 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-GRIA4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of GRIA4 using anti-GRIA4 antibody.Lane 1:Rat Brain Tissue;2:Mouse Brain Tissue.