Ionotropic Glutamate receptor 2/GRIA2 Antibody

• Catalog Number: orb215959
• Category: Antibodies
• Description: Ionotropic Glutamate receptor 2/GRIA2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Predicted Reactivity: Bovine, Equine, Monkey, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human GRIA2 recombinant protein (Position: N25-I360). Human GRIA2 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA2.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat Immunocytochemistry, 0.5-1μg/ml, Human Immunofluorescence, 2μg/ml, Mouse Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 98821 MW
• UniProt ID: P42262
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Glutamate receptor 2;GluR-2;AMPA-selective glutama
• Note: For research use only
• Application notes: WB: The detection limit for GRIA2 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U-87MG cells using anti-GRIA2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of GRIA2 using anti-GRIA2 antibody.Lane 1:rat brain tissue;2:rat C6 cell;3:mouse brain tissue.

IF analysis of GRIA2 using anti-GRIA2 antibody.GRIA2 was detected in paraffin-embedded section of mouse brain tissues.

IF analysis of GRIA2 using anti-GRIA2 antibody. GRIA2 was detected in an immunocytochemical section of T-47D cells.

IHC analysis of GRIA2 using anti-GRIA2 antibody.GRIA2 was detected in paraffin-embedded section of Mouse Brain Tissue.

IHC analysis of GRIA2 using anti-GRIA2 antibody.GRIA2 was detected in paraffin-embedded section of Rat Brain Tissue.

IHC analysis of GRIA2 using anti-GRIA2 antibody. GRIA23 was detected in a frozen section of rat brain tissue.