Interleukin-1beta (IL-1beta) Antibody

• Catalog Number: orb2313495
• Category: Antibodies
• Description: Interleukin-1beta (IL-1beta) Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: IL1B/3993
• Tested applications: ELISA, IHC
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: Recombinant fragment of human IL1B protein (exact sequence is proprietary)
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Dilution range: ELISA (For coating, order antibody without BSA); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 31kDa (proprotein); 17kDa (mature)
• Target: IL1B
• Entrez: 3553
• UniProt ID: P01584
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Catabolin; Hematopoietin 1 (H1); IFN beta inducing
• Note: For research use only
• Application notes: Positive Control: LPS stimulated human peripheral blood mononuclear cells. Human colon or liver tissue
• Expiration Date: 12 months from date of receipt.