integrin beta 4 binding protein/EIF6 Antibody

• Catalog Number: orb312108
• Category: Antibodies
• Description: integrin beta 4 binding protein/EIF6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human EIF6 recombinant protein (Position: N66-T210). Human EIF6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat EIF6.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By HeatImmunocytochemistry, 0.5-1μg/mlWestern blot, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 26599 MW
• UniProt ID: P56537
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Eukaryotic translation initiation factor 6 ;eIF-6
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of EIF6 using anti-EIF6 antibody.Lane 1:human HeLa cell; 2:human K562 cell; 3:human 293T cell; 4:human HepG2 cell; 5:rat liver tissue; 6:mouse liver tissue; 7:mouse RAW264.7 cell.

IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue.

IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in paraffin-embedded section of Mouse Intestine Tissue.

IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in paraffin-embedded section of Rat Testis Tissue.

IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in immunocytochemical section of SMMC-7721 cell.

IHC analysis of EIF6 using anti-EIF6 antibody.EIF6 was detected in immunocytochemical section of SW480 cell.