CD45 Mouse Monoclonal Antibody

SKU: orb3085800

Description

The CD45 Mouse Monoclonal Antibody (Carrier-free) is a Carrier-free antibody raised in mouse. It is immunogen affinity purified and suitable for use in FC, IF, IHC, and WB applications. This antibody targets the Receptor-type tyrosine-protein phosphatase C in Human samples.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Immunology & Inflammation, Metabolism Research, Neuroscience, Pharmacology & Drug Discovery, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution Range: Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
• Reactivity: Human
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: B4J5
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CD45, different from the related mouse sequence by eight amino acids, and from the related rat sequence by ten amino acids.
• Target: Receptor-type tyrosine-protein phosphatase C
• Molecular Weight: 220 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Carrier-free

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B220; CD45; CD45R; GP180; L CA; LCA; Leukocyte common antigen; LY5; PTPRC; RPTPC; T200

Quick Database Links

Gene Symbol

Receptor-type tyrosine-protein phosphatase C

UniProt

P08575