Integrin beta 1/ITGB1 Antibody

• Catalog Number: orb196256
• Category: Antibodies
• Description: Integrin beta 1/ITGB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ITGB1 recombinant protein (Position: N527-D728). Human ITGB1 shares 91% and 88% amino acid (aa) sequences identity with mouse and rat ITGB1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 88415 MW
• UniProt ID: P05556
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Integrin beta-1;Fibronectin receptor subunit beta;
• Note: For research use only
• Application notes: WB: The detection limit for ITGB1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of ITGB1 using anti-ITGB1 antibody.Lane 1:human A431 cell;2:rat C6 cell.

IHC analysis of ITGB1 using anti-ITGB1 antibody. ITGB1 was detected in a paraffin-embedded section of human placenta tissue.

IHC analysis of ITGB1 using anti-ITGB1 antibody. ITGB1 was detected in a paraffin-embedded section of human tonsil tissue.