Integrin beta 1 antibody

• Catalog Number: orb44007
• Category: Antibodies
• Description: Mouse monoclonal antibody to Integrin beta 1
• Clonality: Monoclonal
• Clone Number: MEM-101A
• Tested applications: FC, IP, WB
• Reactivity: Canine, Human, Porcine
• Isotype: Mouse IgG1
• Immunogen: Raji Burkitt's lymphoma cell line
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 2 μg/ml; positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate; non-reducing conditions, 6% separating gel, 4% stacking gel. Flow cytometry: Recommended dilution: 2 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD29
• Entrez: 3688
• UniProt ID: P05556
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti-beta1 integrin antibody, anti-CD29 antibody,
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 2 μg/ml; positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate; non-reducing conditions, 6% separating gel, 4% stacking gel. Flow cytometry: Recommended dilution: 2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD29 (MEM-101A). Lane 1: lysate of human PBL; Lane 2: lysate of canine PBL; Lane 3: lysate of porcine PBL.

Separation of human CD29 positive platelets (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD29 (MEM-101A) purified antibody (concentration in sample 3 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD29 (MEM-101A) purified antibody (concentration in sample 3 µg/ml) GAM APC.