Integrin alpha 4/ITGA4 Antibody

• Catalog Number: orb315156
• Category: Antibodies
• Description: Integrin alpha 4/ITGA4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, IHC-Fr, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin alpha 4 (999-1028aa MWKAGFFKRQYKSILQEENRRDSWSYINS K), different from the related mouse sequence by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 114900 MW
• UniProt ID: P13612
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Integrin alpha-4;CD49 antigen-like family member D
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of THP-1 cells using anti-Integrin alpha 4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Integrin alpha 4 using anti-Integrin alpha 4 antibody.Lane 1:Rat Liver Tissue;2:Rat Kidney Tissue;3:JURKAT Cell;4:22RV1 Cell;5:HEPG2 Cell;6:SMMC Cell.

IHC analysis of Integrin alpha 4 using anti-Integrin alpha 4 antibody. Integrin alpha 4 was detected in a paraffin-embedded section of human mammary cancer tissue.

IHC analysis of Integrin alpha 4 using anti-Integrin alpha 4 antibody.Integrin alpha 4 was detected in frozen section of human placenta tissues.