INSM1 Antibody

• Catalog Number: orb1733233
• Category: Antibodies
• Description: INSM1 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: between 50 and 100
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: INSM1
• UniProt ID: Q01101
• Storage: Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: zinc finger protein IA-1; insulinoma-associated pr
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human INSM1 by western blot. Samples: Whole cell lysate (25 µg) from HEK293T, NCI-H1155, HeLa, SHP-77, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse INSM1 by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3, Beta-TC-6, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human INSM1 by immunohistochemistry. Sample: FFPE section of neuroendocrine tumor. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human INSM1 by immunohistochemistry. Sample: FFPE section of human pancreas. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse INSM1 by immunocytochemistry. Sample: FFPE section of Beta-TC-6 cells. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human INSM1 by immunocytochemistry. Sample: FFPE section of SHP-77 cells. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233). Secondary: HRP-conjugated goat anti-rabbit IgG.