INSIG1 Antibody (N-term)

• Catalog Number: orb1928195
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB21689
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 29987 Da
• Target: This INSIG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the N-terminal region of human INSIG1.
• UniProt ID: O15503
• NCBI: NP_938150.2, NP_938151.1, NP_005533.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Insulin-induced gene 1 protein, INSIG-1, INSIG1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of INSIG1 Antibody (N-term) in Hela cell line lysates (35 ug/lane). INSIG1 (arrow) was detected using the purified Pab.

INSIG1 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

INSIG1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the INSIG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.