INS Antibody

• Catalog Number: orb1252747
• Category: Antibodies
• Description: INS Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2D11-H5 or INS05
• Tested applications: IF, IHC, WB
• Reactivity: Bovine, Human, Porcine
• Isotype: IgG1, kappa
• Immunogen: Full length purified pig Insulin (aa 1-84) was used as the immunogen for this antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. No special pretreatment is required for staining of formalin/paraffin tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: INS
• UniProt ID: P01308
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MO
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. No special pretreatment is required for staining of formalin/paraffin tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of human pancreas stained with Insulin antibody (2D11-H5). Note cytoplasmic staining of cells.

SDS-PAGE Analysis of Purified, BSA-Free Insulin Antibody (clone 2D11-H5 or INS05). Confirmation of Integrity and Purity of the Antibody.