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Catalog Number | orb443189 |
---|---|
Category | Antibodies |
Description | INPPL1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human INPPL1 recombinant protein (Position: R1172-K1258). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 150 kDa |
UniProt ID | O15357 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Phosphatidylinositol 3,4,5-trisphosphate 5-phospha Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of A431 cells using anti-INPPL1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of INPPL1 using anti-INPPL1 antibody.Lane 1:human HeLa cell;2:human K562 cell;3:rat brain tissue;4:mouse brain tissue.
IF analysis of INPPL1 using anti-INPPL1 antibody.INPPL1 was detected in immunocytochemical section of U20S cells.
IHC analysis of INPPL1 using anti-INPPL1 antibody.INPPL1 was detected in paraffin-embedded section of human gastric cancer tissue.
IHC analysis of INPPL1 using anti-INPPL1 antibody.INPPL1 was detected in paraffin-embedded section of human lung cancer tissue.
IHC analysis of INPPL1 using anti-INPPL1 antibody.INPPL1 was detected in paraffin-embedded section of human ovary cancer tissue.
IHC analysis of INPPL1 using anti-INPPL1 antibody.INPPL1 was detected in paraffin-embedded section of human placenta tissue.
IHC analysis of INPPL1 using anti-INPPL1 antibody.INPPL1 was detected in paraffin-embedded section of mouse spleen tissue.
IHC analysis of INPPL1 using anti-INPPL1 antibody.INPPL1 was detected in paraffin-embedded section of rat brain tissue.
IHC analysis of INPPL1 using anti-INPPL1 antibody.INPPL1 was detected in paraffin-embedded section of rat lung tissue.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Bovine, Canine, Equine, Human, Porcine, Rabbit |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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