Topoisomerase II alpha Antibody / TOP2A

• Catalog Number: orb750178
• Category: Antibodies
• Description: This mAb recognizes a 170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Human recombinant partial protein (amino acids 1352-1493) was used as the immunogen for this TOP2A antibody.
• UniProt ID: P11388
• Tested applications: IF, IHC, WB
• Dilution range: Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml (1)
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.1. Staining of FFPE tissue requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
• Antibody Type: Primary Antibody
• Clone Number: TPM2A-1
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This TOP2A antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human tonsil with TOP2A antibody (clone TPM2A-1).

IHC testing of FFPE human bladder carcinoma with TOP2A antibody (clone TPM2A-1).

Immunofluorescent staining of PFA-fixed human HeLa cells with TOP2A antibody (clone TPM2A-1).

Western blot testing of human HeLa cell lysate with TOP2A antibody (clone TPM2A-1). Expected molecular weight ~174 kDa.