STEAP1/STEAP Antibody

• Catalog Number: orb1537960
• Category: Antibodies
• Description: STEAP1/STEAP Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: J2D2
• Tested applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2b,k
• Immunogen: Recombinant human STEAP1 (1-70aa) purified from E. coli. NCBI Accession No: NP_036581.
• Concentration: 1 mg/ml
• Dilution range: ELISA, ICC, IF, IHC, IHC-P (10 µg/ml), WB (1:500 - 1:1000)
• Conjugation: Unconjugated
• Target: STEAP1 / STEAP
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol. PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
• Alternative names: STEAP1, Metalloreductase STEAP1, PRSS24, STEAP
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B3003 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3003 was determined to be 10 ug/ml.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of human LNCap cell colony with monoclonal antihuman STEAP1 antibody