SARS-CoV-2 (COVID-19) Membrane Antibody

• Catalog Number: orb1239082
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Membrane Antibody
• Target: M
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Virus
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) Membrane Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: SARS-CoV-2 (COVID-19) Membrane Antibody is affinity chromatography purified via peptide column.
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Membrane antibody (orb1239082) was raised against a peptide corresponding to 15 amino acids near the carboxyl terminus of SARS-CoV-2 (COVID-19) Membrane protein. The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19) Membrane protein.
• Tested applications: ELISA, IHC, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SARS-CoV-2/SARS-CoV Matrix Antibody: SARS-CoV-2 Ma
• Research Area: Infectious Disease & Virology
• Note: For research use only
• NCBI: QJW69073

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Membrane in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Membrane antibody (orb1239082, 0.5 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Membrane protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.

ELISA Validation with SARS-CoV-2 (COVID-19 Membrane Protein. Antibodies: SARS-CoV-2 (COVID-19) Membrane Antibody, orb1239082. A direct ELISA was performed using SARS-COV-2 membrane protein (orb1231561) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Membrane antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 3000 ng/mL.

Western Blot Validation with SARS-CoV-2 (COVID-19) Membrane Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) membrane protein (orb1231561). Antibodies: SARS-CoV-2 (COVID-19) membrane, orb1239082, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 µg/mL and. Lane 2: 0.5 µg/mL.