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RNF166 Antibody (Center)
Catalog Number: orb1927341
| Catalog Number | orb1927341 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, WB |
| Predicted Reactivity | Rat |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Dilution range | IF: 1:25, WB: 1:1000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 26122 Da |
| Target | This RNF166 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-97 amino acids from the Central region of human RNF166. |
| UniProt ID | Q96A37 |
| NCBI | NP_001165286.1, NP_001165287.1, NP_849163.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | RING finger protein 166, RNF166 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
RNF166 Antibody (Center) western blot analysis in 293, mouse NIH/3T3 cell line and testis tissue lysates (35 ug/lane).This demonstrates the RNF166 antibody detected the RNF166 protein (arrow).
Fluorescent image of NIH/3T3 cells stained with RNF166 Antibody (Center). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
