PRMT5 Recombinant Rabbit Monoclonal Antibody

SKU: orb1172977

Description

PRMT5 Recombinant Rabbit Monoclonal Antibody

Research Area

Arginine Methylation, DNA and RNA, Epigenetics and Nuclear Signaling

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-1000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200, Flow-Cyt=1:50-100
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG/Kappa
• Clone No.: D1B12
• Target: PRMT5
• Molecular Weight: 70 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ANM5_HUMAN; Protein arginine N-methyltransferase 5; HRMT1L5; IBP72; JBP1; SKB1; EC:2.1.1.320; 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog(SKB1 homolog; SKB1Hs); Protein arginine N-methyltransferase 5, N-terminally processed; protein arginine methyltransferase 5; HSL7; SKB1Hs;

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:10000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: HEK-293, 3: MCF7, 4: HepG2, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 73 kDa, Observed MW: 70 kDa.

Tissue: Human breast cancer, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172977.

Tissue: Human liver, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172977.

Quick Database Links

Gene Symbol

PRMT5