Pig B-Cell Activating Factor (BAFF/CD257) ELISA Kit

• Catalog Number: orb782106
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell Activating Factor(BAFF).
• Reactivity: Porcine
• Concentration: 10 ng/mL
• Tested applications: ELISA
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig BAFF/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig BAFF/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig BAFF/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig BAFF/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Alternative names: CD257 ELISA Kit, TNFSF13B ELISA Kit, BLYS ELISA Ki
• Research Area: Cancer Biology, Immunology & Inflammation, Infecti
• Note: For research use only
• Expiration Date: Please enquire.