Phospho-Fos B (Ser27) Rabbit Polyclonal Antibody

• Catalog Number: orb156921
• Category: Antibodies
• Description: Phospho-Fos B (Ser27) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Fos B around the phosphorylation site of Ser27 QYL(p-S)SV
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 36 kDa
• Target: FOSB
• UniProt ID: P53539
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Fos B(phospho S27); p-Fos B(phospho S27); p-FosB(p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Hela (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Polycystin 1 antibody (orb156921), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Raji (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Fos B (Ser27) (orb156921) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 35 kD.

Sample: Siha (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Fos B (Ser27) (orb156921) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 35 kD.