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PD-L1 Protein, Human, Recombinant (mFc)

PD-L1 Protein, Human, Recombinant (mFc)

Catalog Number: orb1955105

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb1955105
CategoryProteins
DescriptionPD-L1 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 51.7 kDa and the accession number is Q9NZQ7-1.
TagC-mFc
Purity98.00%
MW51.7 kDa (predicted); 66 kDa (reducing condition, due to glycosylation)
UniProt IDQ9NZQ7
Expression SystemHEK293 Cells
Biological OriginHuman
Biological ActivityPD-L1 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 51.7 kDa and the accession number is Q9NZQ7-1.
Expression RegionA DNA sequence encoding the human CD274 (NP_054862.1) (Met1-Thr239) was expressed with the Fc region of mouse IgG1 at the C-terminus. Predicted N terminal: Phe 19
Storage-20°C
NoteFor research use only
Application notesA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Expiration Date6 months from date of receipt.
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    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide.

    Mammalian

    100 μg, 10 μg, 50 μg
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    > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC

    Due to glycosylation, the protein migrates to 63-68 kDa based on Tris-Bis PAGE result.

    100 μg
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    ELISA,  MS,  SDS-PAGE,  WB

    Greater than 95% as determined by reducing SDS-PAGE.

    Human cells

    50 μg, 500 μg, 10 μg
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