PD-L1 antibody

SKU: orb2276605

Description

PD-L1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Alveolar macrophages, tonsil crypt epithelium and the placental cytotrophoblast show strong, predominantly membranous positivity. Follicle centre macrophages should show at least moderate intensity staining. Negative Control: Appendix (negative staining of all epithelial cells) Cellular Localization: Predominantly membranous Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: Biorbyt-711R
• Immunogen: KLH-conjugated linear peptide corresponding to human PD-L1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1

Breast Breast cancer NST with PD L1 negative tumor cells but intense PD L1 staining in tumor associated inflammatory cells.

Colon Colorectal adenocarcinoma with moderate to strong membranous PD L1 staining in the vast majority of its tumor cells.

In the placenta, PD-L1 staining is predominantly seen at the surface membrane of the syncytiotrophoblast.

Quick Database Links

UniProt

Q9NZQ7