PCCB Recombinant Mouse Monoclonal Antibody

SKU: orb1172477

Description

PCCB Recombinant Mouse Monoclonal Antibody

Research Area

Amino Acid Metabolism, Cancer Research, Cell Biology, Energy Metabolism

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG2a, Kappa
• Clone No.: B10B10
• Target: PCCB
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: room temperature 2h, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: Human liver, 2: HepG2, 3: Rat liver, 4: Mouse heart, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 58 kDa, Observed MW: 55 kDa.

Tissue: Human heart, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172477.

Quick Database Links

Gene Symbol

PCCB