NCAM/CD56 Antibody

• Catalog Number: orb1537023
• Category: Antibodies
• Description: NCAM/CD56 Antibody
• Target: NCAM / CD56
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Human, Rat
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence C-KASWTRPEKQETLD, from the internal region of the protein sequence according to NP_851996.2.
• Tested applications: ELISA, IHC-P, WB
• Dilution range: IHC-P (5 µg/ml), Peptide-ELISA (1:32000), WB (0.01 - 0.03 µg/ml)
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 170kD band observed in Human Brain (Cerebellum, Cerebral and Frontal Cortex) lysates (calculated MW of 94.6kD according to NP_851996.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.01-0.03 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NCAM1, CD56, CD56 antigen, MSK39, NCAM-1, N-CAM-1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

NCAM1 antibody (0.01 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.