NCAM / CD56 Antibody

SKU: orb534487

Description

Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  The optimal dilution of the CD56 antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: 56C04, also called 123A8
• Immunogen: A membrane preparation of small cell lung carcinoma was used as the immunogen for this CD56 antibody. This mAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot testing of human brain tissue with CD56 antibody (clone 56C04/123A8). Predicted molecular weight: ~110 kDa (soluble fragment), ~120/125 kDa (GPI-anchored), 140/180 kDa (transmembrane isoforms).

IHC staining of FFPE human pancreas tissue with CD56 antibody (clone 56C04/123A8). HIER: boil tissue sections in pH9 EDTA buffer for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human thyroid tissue with CD56 antibody (clone 56C04/123A8). HIER: boil tissue sections in pH9 EDTA buffer for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human colon tissue with CD56 antibody (clone 56C04/123A8). HIER: boil tissue sections in pH9 EDTA buffer for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free CD56 antibody (clone 56C04/123A8) as confirmation of integrity and purity.

Quick Database Links

UniProt

P13591