Epithelial Marker Antigen Antibody / MUC1 / Mucin-1

SKU: orb385658

Description

This Epithelial Membrane Antigen / EMA antibody, also called MUC1 and Mucin-1, recognizes the full-length protein in a glycosylation-independent manner and can bind to the fully glycosylated protein. The dominant epitope of this mAb is APDTR in the VNTR region. It reacts with the core peptide of the EMA protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the mAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This EMA antibody reacts with both normal and malignant epithelia of various tissues including breast and colon.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Epithelial Marker Antigen antibody to be titered up or down for optimal performance.1. FFPE staining REQUIRES boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 10-20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: MUC1/967
• Immunogen: Human milk fat globule membranes were used as the immunogen for this Epithelial Marker Antigen antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot testing of human MCF7 cell lysate with Epithelial Marker Antigen antibody (clone MUC1/967). This glycoprotein is commonly visualized between 120~500 kDa.

Epithelial Marker Antigen antibody IHC testing of formalin-paraffin human endometrial cancer tissue (clone MUC1/967).

Epithelial Marker Antigen antibody IHC testing of formalin-paraffin human breast cancer tissue (clone MUC1/967).

Epithelial Marker Antigen antibody IHC testing of formalin-paraffin human ovarian cancer tissue (clone MUC1/967).