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Catalog Number | orb257762 |
---|---|
Category | Proteins |
Description | Mouse PD-L1, His Tag is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Thr 238 (Accession # NP_068693). |
Reactivity | Mouse |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 26.6 kDa |
Target | PD-L1, B7-H1 |
UniProt ID | Q9EP73 |
Protein Sequence | NP_068693 |
Source | Mouse PD-L1, His Tag (orb257762) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Thr 238 (Accession # NP_068693). |
Expression System | HEK293 |
Biological Origin | Mouse |
Expression Region | Phe 19 - Thr 238 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_068693 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Mouse PD-L1 protein
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide. | |
Mammalian |
98.00% | |
28.02 kDa (predicted); 46.7 kDa (reducing conditions) |
98.00% | |
26.2 kDa (predicted) |
SDS-PAGE: 97.2%; SEC-HPLC: 91.5% | |
26.3 kDa (predicted); 43-55 kDa (reducing condition, due to glycosylation) |