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Catalog Number | orb1958466 |
---|---|
Category | Proteins |
Description | PD-L1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.3 kDa and the accession number is Q3U472. |
Tag | C-His |
Purity | SDS-PAGE: 97.2%; SEC-HPLC: 91.5% |
MW | 26.3 kDa (predicted); 43-55 kDa (reducing condition, due to glycosylation) |
UniProt ID | Q3U472 |
Expression System | HEK293 Cells |
Biological Origin | Mouse |
Biological Activity | PD-L1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.3 kDa and the accession number is Q3U472. |
Expression Region | A DNA sequence encoding the mouse CD274 (NP_068693.1) extracellular domain (Met 1-Thr 238) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Phe 19 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result. |
> 90% as determined by SDS-PAGE | |
26.8 kDa |
98.00% | |
28.02 kDa (predicted); 46.7 kDa (reducing conditions) |
98.00% | |
26.2 kDa (predicted) |