IDE Antibody (Center)

SKU: orb1935700

Description

IDE Antibody (Center)

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 117968 Da

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Western blot analysis of anti-IDE Antibody (Center) in A375 cell line lysates (35 ug/lane). IDE (arrow) was detected using the purified Pab.

Western blot analysis of lysate from K562 cell line, using IDE Antibody (Center). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-IDE Antibody (Center) at 1:2000 dilution. Lane 1: A375 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 118 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-IDE Antibody (Center) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: A375 whole cell lysate. Lane 4: K562 whole cell lysate. Lane 5: mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 118 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P14735