ICAM-1 Antibody / CD54

SKU: orb639471

Description

Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 mAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This mAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).

Images & Validation

• Tested Applications: FA, FACS, IHC-P
• Dilution range: Flow cytometry: 0.5-1ug/million cells in 0.1ml,Functional testing (order BSA/sodium azide-free format),Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
• Reactivity: Human
• Application Notes:
  Optimal dilution of the ICAM-1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: 1H4 or W-CAM-1 or Wehi-CAM-1
• Immunogen: Raji Burkitt lymphoma cells were used as the immunogen for the ICAM-1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC analysis of formalin-fixed, paraffin-embedded human melanoma stained with ICAM-1 antibody (clone 1H4).

Western blot testing of human Raji cell lysate with ICAM-1 antibody (clone 1H4). Predicted molecular weight: ~58/75-115kDa (unmodified/glycosylated).

SDS-PAGE Analysis of Purified, BSA-Free ICAM-1 Antibody (clone 1H4 or W-CAM-1 or Wehi-CAM-1). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P05362