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Human Thrombopoietin Protein

Catalog Number: orb867314

DispatchUsually dispatched within 5-10 working days
$ 430.00
Catalog Numberorb867314
CategoryProteins
DescriptionHuman Thrombopoietin Protein, Tag Free is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Gly 353 (Accession # P40225-1).
ReactivityHuman
TagNative
Form/AppearancePowder
Purity95%
ConjugationUnconjugated
MW35.6 kDa
TargetThrombopoietin, TPO
UniProt IDP40225
SourceHuman Thrombopoietin, premium grade (orb867314) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Gly 353 (Accession # P40225-1).
Expression SystemHEK293
Biological OriginHuman
Biological ActivityHuman Thrombopoietin R, His Tag captured on CM5 Chip via anti-His antibody can bind Human Thrombopoietin, premium grade with an affinity constant of 14.9 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Expression RegionSer 22 - Gly 353
Endotoxins0.1 EU per μg
NCBINP_000451.1
Storage-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Buffer/PreservativesPBS, pH7.4
Alternative namesTHPO,MGC163194,MGDF,MKCSF,ML,MPLLG,TPO,THCYT1
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NoteFor research use only
Application notesThis protein carries no "tag". The protein has a calculated MW of 35.6 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expiration Date6 months from date of receipt.
Human Thrombopoietin Protein

Human Thrombopoietin R, His Tag (Cat. No. orb1101194) captured on CM5 Chip via anti-His antibody can bind Human Thrombopoietin, premium grade (Cat. No. orb867314) with an affinity constant of 14.9 nM as determined in SPR assay (Biacore T200) (Routinely tested).

  • Human THPO protein [orb594751]

    Greater than 95% as determined by SDS-PAGE.

    37.3 kDa

    Mammalian cell

    10 μg, 50 μg, 1 mg, 500 μg
  • Human MPL protein [orb244381]

    Greater than 90% as determined by SDS-PAGE.

    56.5 kDa

    E.coli

    20 μg, 100 μg, 1 mg
  • Human MPL protein [orb245925]

    Greater than 90% as determined by SDS-PAGE.

    54.5 kDa

    Yeast

    1 mg, 20 μg, 100 μg
  • Human TPO (N, C-6His) Protein [orb1471766]

    Greater than 95% as determined by reducing SDS-PAGE.

    37.3 KDa

    Mammalian

    10 μg, 50 μg
  • Human THPO protein [orb604411]

    Greater than 85% as determined by SDS-PAGE.

    34.7 kDa

    E.coli

    20 μg, 100 μg, 1 mg