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Human THPO protein

Catalog Number: orb594751

DispatchUsually dispatched within 1-2 weeks
$ 340.00
Catalog Numberorb594751
CategoryProteins
DescriptionRecombinant Human Thrombopoietin(THPO) (Active)
TagN-terminal 6xHis-tagged and C-terminal 6xHis-tagged
Form/AppearanceLyophilized powder
PurityGreater than 95% as determined by SDS-PAGE.
MW37.3 kDa
UniProt IDP40225
Protein SequenceSPAPPACDLRVLSKLLRDSHVLHSRLSQCPEVHPLPTPVLLPAVDFSLGEWKTQMEETKAQDILGAVTLLLEGVMAARGQLGPTCLSSLLGQLSGQVRLLLGALQSLLGTQLPPQGRTTAHKDPNAIFLSFQHLLRGKVRFLMLVGGSTLCVRRAPPTTAVPSRTSLVLTLNELPNRTSGLLETNFTASARTTGSGLLKWQQGFRAKIPGLLNQTSRSLDQIPGYLNRIHELLNGTRGLFPGPSRRTLGAPDISSGTSDTGSLPPNLQPGYSPSPTHPPTGQYTLFPLPPTLPTPVVQLHPLLPDPSAPTPTPTSPLLNTSYTHSQNLSQEG
Protein LengthFull Length of Mature Protein
SourceMammalian cell
Biological OriginHomo sapiens (Human)
Biological ActivityThe ED50 as determined in a cell proliferation assay using MO7E human megakaryocytic leukemic cells is 0.55 ng/ml.
Expression Region22-353aa
EndotoxinsLess than 1.0 EU/µg as determined by LAL method.
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Buffer/PreservativesLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 150 mM NaCl, pH 8.0
Alternative namesThrombopoietin;C-mpl ligand;Megakaryocyte colony-s
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NoteFor research use only
Application notesFull Length of Mature Protein
Expiration Date6 months from date of receipt.
Human THPO protein

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

  • Human Thrombopoietin Protein [orb867314]

    Unconjugated

    95%

    35.6 kDa

    Human Thrombopoietin, premium grade (orb867314) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Gly 353 (Accession # P40225-1).

    1 mg, 50 μg, 20 μg
  • Human TPO (N, C-6His) Protein [orb1471766]

    Greater than 95% as determined by reducing SDS-PAGE.

    37.3 KDa

    Mammalian

    10 μg, 50 μg
  • Human THPO protein [orb604411]

    Greater than 85% as determined by SDS-PAGE.

    34.7 kDa

    E.coli

    20 μg, 100 μg, 1 mg
  • Recombinant human TPO protein (Active, HEK293) [orb1817180]

    > 95% as determined by SDS-PAGE

    80-85 kDa

    100 μg, 50 μg, 10 μg
  • Human THPO protein [orb392562]

    ELISA,  MS,  SDS-PAGE,  WB

    Greater than 95% as determined by reducing SDS-PAGE.

    Human cells

    500 μg, 50 μg, 10 μg