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Catalog Number | orb392562 |
---|---|
Category | Proteins |
Description | Recombinant human THPO protein is produced by our mammalian expression system and the target gene encoding Ser22-Gly353 is expressed with a 6His tag at the N-terminus, 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | THPO |
Entrez | 7066 |
UniProt ID | P40225 |
Protein Sequence | HHHHHHSPAP PACDLRVLSK LLRDSHVLHS RLSQCPEVHP LPTPVLLPAV DFSLGEWKTQ MEETKAQDIL GAVTLLLEGV MAARGQLGPT CLSSLLGQLS GQVRLLLGAL QSLLGTQLPP QGRTTAHKDP NAIFLSFQHL LRGKVRFLML VGGSTLCVRR APPTTAVPSR TSLVLTLNEL PNRTSGLLET NFTASARTTG SGLLKWQQGF RAKIPGLLNQ TSRSLDQIPG YLNRIHELLN GTRGLFPGPS RRTLGAPDIS SGTSDTGSLP PNLQPGYSPS PTHPPTGQYT LFPLPPTLPT PVVQLHPLLP DPSAPTPTPT SPLLNTSYTH SQNLSQEGVD HHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
Alternative names | MGDF; Thrombopoietin; C-mpl ligand; ML; Megakaryoc Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 95% as determined by SDS-PAGE. | |
37.3 kDa | |
Mammalian cell |
Unconjugated | |
95% | |
35.6 kDa | |
Human Thrombopoietin, premium grade (orb867314) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Gly 353 (Accession # P40225-1). |
Greater than 95% as determined by reducing SDS-PAGE. | |
37.3 KDa | |
Mammalian |
Greater than 85% as determined by SDS-PAGE. | |
34.7 kDa | |
E.coli |
> 95% as determined by SDS-PAGE | |
80-85 kDa |