Human Neurokinin A (NKA) ELISA Kit

SKU: orb778174

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Neurokinin A(NKA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neurokinin A(NKA).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NKA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 4.96 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

NK-A ELISA Kit, SK ELISA Kit, Substance K ELISA Kit, Neuropeptide K ELISA Kit, Neurokinin K ELISA Kit, Neuromedin L ELISA Kit, Neuropeptide gamma ELISA Kit