Human Neurokinin A (NKA) ELISA Kit

SKU: orb1946842

Description

Human Neurokinin A (NKA) ELISA Kit

Images & Validation

• Application Notes:
  This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human NKA. During the reaction, Human NKA in the sample or standard competes with a fixed amount of Human NKA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human NKA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human NKA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, Tissue homogenate and Other biological samples
• Assay Type: Competitive
• Assay Time: 1.5H
• Range: 15.63-1000pg/mL
• Sensitivity: 9.38 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

NK-A, SK, Substance K, Neuropeptide K, Neurokinin K, Neuromedin L, Neuropeptide gamma

Quick Database Links

Gene Symbol

NKA

UniProt

P20366