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Human IL12B Protein, Fc Tag
Description
Images & Validation
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Key Properties
−| Source | This protein contains the p40 subunit of human IL12, termed asIL12B (NP_0021782) (Met 1-Ser 328) was fused with the Fc region ofhuman IgG1 at the C-terminus and expressed from HEK293 Cells. |
|---|---|
| Expression System | HEK293 Cells |
| Target | IL12B |
| Reactivity | Human |
| Purity | >95%, determined by SDS-PAGE |
| Endotoxins | >95%, determined by SDS-PAGE |
Storage & Handling
−| Disclaimer | For research use only |
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Alternative Names
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 48.7 and 35.5 kDa after removal of the signal peptide.
Mammalian
50 μg, 10 μg, 100 μgHuman IL23A and IL12B Heterodimer Protein, hFc Tag and His Tag [orb1743335]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 44.8 and 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL23A-hFc and IL12B-His is approximately 35-55 kDa due to glycosylation.
E.coli
10 μg, 50 μg, 100 μgHuman IL12B Protein, Fc Tag [orb2697432]
>97%, determined by SDS-PAGE
This protein contains the p40 subunit of human IL12, termed asIL12B (NP_0021782) (Met 1-Ser 328) was fused with the Fc region ofhuman IgG1 at the C-terminus and expressed from HEK293 Cells.
50 μg, 20 μgHuman IL12B Protein, Fc Tag [orb2697435]
>95%, determined by SEC-HPLC
This protein contains the p40 subunit of human IL12, termed asIL12B (NP_0021782) (Met 1-Ser 328) was fused with the Fc region ofhuman IgG1 at the C-terminus and expressed from HEK293 Cells.
100 μg, 20 μgHuman IL12A & IL27B Protein, His & Fc Tag [orb2698055]
>(59.8+33.3)%, determined by SDS-PAGE
This protein contains the p35 subunit of human IL12, termed as IL12A (NP_0008732) (Met1-Ser219) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; This protein contains the human IL27B, (NP_0057462) (Met1-Lys229) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2 . The two plasmids were co-expressed and the heterodimer was purified and expressed from HEK293 Cells.
100 μg, 20 μg

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Human IL12B Protein, Fc Tag (orb2697444)
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