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Catalog Number | orb2697435 |
---|---|
Category | Proteins |
Description | Human IL12B Protein, Fc Tag |
Target | IL12B |
Reactivity | Human |
Purity | > 95%, determined by SEC-HPLC |
Expression System | HEK293 Cells |
Endotoxins | > 95%, determined by SEC-HPLC |
Source | This protein contains the p40 subunit of human IL12, termed asIL12B (NP_0021782) (Met 1-Ser 328) was fused with the Fc region ofhuman IgG1 at the C-terminus and expressed from HEK293 Cells. |
Alternative names | IL12B, interleukin 12B, CLMF, NKSF, CLMF2, IMD28, Read more... |
Note | For research use only |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 48.7 and 35.5 kDa after removal of the signal peptide. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 44.8 and 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL23A-hFc and IL12B-His is approximately 35-55 kDa due to glycosylation. | |
E.coli |
> 97%, determined by SDS-PAGE | |
This protein contains the p40 subunit of human IL12, termed asIL12B (NP_0021782) (Met 1-Ser 328) was fused with the Fc region ofhuman IgG1 at the C-terminus and expressed from HEK293 Cells. |
> 95%, determined by SDS-PAGE | |
This protein contains the p40 subunit of human IL12, termed asIL12B (NP_0021782) (Met 1-Ser 328) was fused with the Fc region ofhuman IgG1 at the C-terminus and expressed from HEK293 Cells. |
> (59.8+33.3)%, determined by SDS-PAGE | |
This protein contains the p35 subunit of human IL12, termed as IL12A (NP_0008732) (Met1-Ser219) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; This protein contains the human IL27B, (NP_0057462) (Met1-Lys229) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2 . The two plasmids were co-expressed and the heterodimer was purified and expressed from HEK293 Cells. |