Human IgG Fc Antibody Alkaline Phosphatase Conjugated

SKU: orb347299

Description

Human IgG F(c) antibody (Alkaline Phosphatase)

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Reactivity: Human
• Application Notes:
  Anti-Human IgG F(c) Alkaline Phosphatase Conjugated antibody has been tested by dot blot and ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Human IgG F(c) was produced by repeated immunization with Human Fc fragment in rabbit.
• Purity: Anti-Human IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab).

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.05% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Human Fc Antibody, Rb-a-Human F(c), Human IgG F(c) Antibody in Rabbit, rabbit anti-Human IgG F(c) Alkaline phosphatase Secondary Antibody

Dot Blot of Human IgG Fc Antibody Alkaline Phosphatase Conjugated. Antigen: Human IgG Fc Load: Lane 1 - 100 ng Lane 2 - 33.33 ng Lane 3 - 11.11 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng Primary antibody: n/a Secondary antibody: Human IgG Fc Antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 60 min at RT. Visualized using Alkaline Phosphatase Substrate.