Human IgG Fc Antibody Alkaline Phosphatase Conjugated

SKU: orb347233

Description

Human IgG F(c) antibody (Alkaline Phosphatase)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:40,000, IHC: 1:200 - 1:1,000, WB: 1:1,000 - 1:5,000
• Reactivity: Human
• Application Notes:
  Anti-Human IgG F(c) Alkaline Phosphatase Conjugated has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Human IgG F(c) fragment
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab).

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

goat anti-Human IgG F(c) alkaline phosphatase Conjugated Antibody, goat anti-Human IgG F(c) fragment antibody alk phos conjugation

ELISA Results of purified Goat Anti-Human IgG F(c) Antibody Alkaline Phosphatase Conjugated tested against purified Human IgG F(c) Alk Phos. Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n orb346220). The working dilution is 1:121000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Alkaline Phosphatase Conjugate Buffer, NPP Working Buffer, and TMB substrate (p/n orb348651).