Human IFN-alpha 1 Protein, Fc Tag

• Catalog Number: orb1147003
• Category: Proteins
• Description: Human IFN-alpha 1, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 189 (Accession # P01562-1).
• Target: IFN-alpha 1
• Conjugation: Unconjugated
• Tag: C-Fc
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH 7.5
• Purity: 95%
• UniProt ID: P01562
• MW: 45.8 kDa
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 45.8 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Expression Region: Cys 24 - Glu 189
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Cytokines
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Loaded Human IFN-alpha / beta R1, His Tag (Cat. No. orb867302) on NTA Biosensor, can bind Human IFN-alpha 1, Fc Tag (Cat. No. orb1147003) with an affinity constant of 11.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).