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Catalog Number | orb383022 |
---|---|
Category | Proteins |
Description | Recombinant human CSF2 protein. |
Tag | N-terminal 6xHis-tagged |
Form/Appearance | Liquid or Lyophilized powder |
Purity | Greater than 90% as determined by SDS-PAGE. |
MW | 16.5 kDa |
UniProt ID | P04141 |
Protein Sequence | APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE |
Protein Length | Full Length of Mature Protein |
Source | Yeast |
Biological Origin | Homo sapiens (Human) |
Expression Region | 18-144aa |
Endotoxins | Not test. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Alternative names | Colony-stimulating factor Short name, CSF Molgramo Read more... |
Note | For research use only |
Application notes | E.coli and Yeast N-terminal 6xHis-tagged Full Length |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 21.07-35.48 pg/mL.
> 98% as determined by SDS-PAGE and HPLC. | |
14.5 kDa | |
E.Coli |
Unconjugated | |
95% | |
14.5 kDa | |
Human GM-CSF, premium grade (orb257510) is expressed from human 293 cells (HEK293). It contains AA Ala 18 - Glu 144 (Accession # NP_000749.2). |
Greater than 95% as determined by SDS-PAGE. | |
14.6 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
15.5 kDa | |
Mammalian cell |
IF, WB | |
Human | |
Mouse | |
Polyclonal | |
Unconjugated |