Human Cluster of Differentiation 200 (CD200) ELISA Kit

SKU: orb777412

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 200(CD200).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD200. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD200. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD200, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD200 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 12.9 pg/mL
• Concentration: 2000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

MOX1 ELISA Kit, MOX2 ELISA Kit, MRC ELISA Kit, OX-2 ELISA Kit, My033 ELISA Kit, Antigen Identified By Monoclonal Antibody MRC OX-2 ELISA Kit

Quick Database Links

UniProt

P41217